Reconstitution and concentration in the laboratory

Lyophilised peptide is supplied as a freeze-dried solid because it is far more stable that way. To work with it in solution, a solvent is added and the solid dissolves. That is reconstitution.

The usual solvent for research handling is bacteriostatic water: sterile water containing 0.9% benzyl alcohol as a preservative. The preservative is what allows a multi-use vial to hold up in a refrigerator for around 28 days instead of a few days.

Concentration is mass divided by volume. Add 2ml of solvent to a 10mg vial and the solution is 5mg per ml. Add 1ml to the same vial and it is 10mg per ml. That is the whole calculation.

The choice of volume is a laboratory decision about the working range of the equipment and the precision required. A more dilute solution makes small volumes easier to measure accurately. A more concentrated one makes the vial last longer.

The MYOGENX concentration calculator does this arithmetic. It is a laboratory tool for expressing a prepared solution, and it is not dosing guidance of any kind.

Bring the vial and the solvent to room temperature first. Cold glass condenses atmospheric moisture into material that needs to stay dry.

Run the solvent slowly down the inner wall of the vial. Do not fire it directly into the lyophilised cake.

Do not shake. Agitation at the air-liquid interface denatures peptides. Roll the vial gently between the fingers and let it dissolve in its own time.

Aliquot immediately into single-use portions and freeze them. This is what protects the material from cumulative freeze-thaw damage.

MYOGENX cannot provide guidance relating to human consumption, including dosage, protocols or administration, under any circumstances. We operate strictly as a research supplier.

Everything above concerns getting a solid into solution and knowing the concentration of that solution. Those are laboratory handling questions. Anything beyond that is outside what we supply and outside what we will advise on.